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Related post: Research Microbiologist LMSF NIAID (Retired) Medical Director EB NIAID Scientist LMSF NIAID COOPERATING UNITS (if any) None lab/branch Laboratory of Microbial Structure and Function, Hamilton, MT 59840 SECTION Rickettsial Diseases Section INSTITUTE AND LOCATION NIAID, NIH, Bethesda, MD 20205 TOTAL MANYEARS: 0.7 PROFESSIONAL: 0.4 OTHER: 0.3 CHECK APPflOPRIATE BOX(ES) D (a) HUMAN SUBJECTS D (al) MINORS Q (a2) INTERVIEWS n (b) HUMAN TISSUES (c) NEITHER SUMMARY OF WORK (200 words or less - underline keywords) The goal of this study is the characterization of those rickettsial constituents which permit Rickettsia rickettsii to successfully invade and multiply within the tissues of man and laboratory animals. Six strains of R_. rickettsii were classified into three virulence groups on the basis of the ability of the rickettsiae to produce disease in guinea pigs. Differences in representatives of these virulence classes were demonstrated by ra^ioimmune precipitation of extracts of the rickettsiae surface-labeled with I. 27-20 PMS-6040 SMITHSONIAN SCIENCE INFORMATION EXCHANGE PROJECT NUMBER (Do NOT use this space) U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00231-01 LMSF PERIOD COVERED October 1, 1981, to September 30, 1982 TITLE OF PROJECT (80 characters or less) Biology of Relapsing Fever Borrelia spp. NAMES, LABORATORY AND INSTITUTE AFFILIATIONS, AND TITLES OF PRINCIPAL INVESTIGATORS AND ALL OTHER PROFESSIONAL PERSONNEL ENGAGED Flutamide Eulexin ON THE PROJECT PI: A. G. Barbour OTHER: R. C. Judd L. M. Mayer H. G. Stoenner Senior Staff Fellow Staff Fellow Expert (Microbiology) (Retired) LMSF NIAID LMSF NIAID LMSF NIAID COOPERATING UNITS (if any) None lab/branch Laboratory of Microbial Structure and Function, Hamilton, MT 59840 SECTION INSTITUTE AND LOCATION NIAID, NIH, Bethesda, MD 20205 TOTAL MANYEARS: 0.9 PROFESSIONAL: 0.4 0.5 CHECK APPROPRIATE BOX(ES) n (a) HUMAN SUBJECTS n (al) MINORS □ (a2) INTERVIEWS n (b) HUMAN TISSUES ^ (c) NEITHER SUMMARY OF WORK (200 words or less - underline keywords) Borreliae undergo antigenic variation during the course of relapsing fever infection. Several serotypes are found among the progeny of a single borrelia inoculated into an experimental animal. We are studying the mechanism of anti- genic shifts in these spirochetes . Our analysis of Borrelia hermsii lysates with sodium dodecyl sulfate-polyacrylamide gel electrophoresis has revealed a major protein that varies in apparent molecular weight between serotypes. Using both polyclonal antisera and monoclonal antibodies, we have shown that these variable proteins are Buy Eulexin also sero type-specif ic antigens . We are purifying the variable proteins for further biochemical characterization and employing recombinant DNA techniques to identify the gene or genes involved in antigenic variation. 27-21 PHS-6040 SMITHSONIAN SCIENCE INFORMATION EXCHANGE PROJECT NUMBER (Do NOT use this space; U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES PUBLIC HEALTH SERVICE
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